i-ejenti yezimpande eyenziwe nge-6-Benzylaminopurine (6-BA) kanye ne-Naphthyl Acetic Acid (NAA) isilawuli sokukhula kwesitshalo esihlanganisa imisebenzi yama-cytokinins nama-auxins, ekhuthaza ukuthuthukiswa kwezimpande nokukhula kwezitshalo ngokusebenzisa isenzo se-synergistic.
Indlela Yesenzo Nemiphumela: Kulokhu kwakhiwa, i-6-Benzylaminopurine, njenge-cytokinin, ikhuthaza ngokuyinhloko ukuhlukana kwamangqamuzana nokuhlukaniswa, ibambezeleka ukubukeka kwamaqabunga, futhi inikeza ukusekelwa okunomsoco ukuze kususwe izimpande. I-Naphthyl Acetic Acid, njenge-analogue ye-auxin, ivuselela ukuhlukaniswa kwamaseli we-sheath yangaphakathi ezimpandeni, ibangele ukwakheka kwezimpande okufunayo. Ukuhlanganiswa kwalokhu okubili kuthuthukisa umphumela we-rooting, ikakhulukazi ngokusheshisa ukuhlukaniswa kwezimpande, ukwandisa inani lezimpande ezihlangene, ukuthuthukisa izinga lokusinda lokusikwa, futhi kuthuthukise ukuqina okuphelele kwesitshalo, okwenza kube kulungele ukusakazeka kwezitshalo okunzima ukuzisusa. Izimo ezijwayelekile zohlelo kanye nezindlela zokusebenzisa:
Izihlahla Zezithelo Nezihlahla Zamahlathi: Ekusakazweni kwamagilebhisi, ubhabhulini, njll., ngokusikwa, umkhuba ovamile ukucwilisa isisekelo se-cuttings ku-5% isisombululo sempuphu encibilikayo ehlanjululwe izikhathi ezingu-250-500 amahora angu-8-12 ukuze kuthuthukiswe ukuhluma nokuhluma.
Imifino Nezimbali: Ngemva kokutshala kabusha ukhukhamba, isisombululo samanzi esingu-1.05% esihlanjululwe izikhathi ezingu-4000-6000 singafafazwa; noma elayisi esigabeni seqabunga elilodwa-inhliziyo eyodwa, isisombululo sempushana esimanzi esingu-50% esihlanjululwe izikhathi ezingu-25000-33000 singafuthwa ukuze sifinyeze isikhathi sokusungulwa kwezithombo futhi sithuthukise ukumelana nokucindezeleka.
Isiko Lezicubu: Kusikompilo le-in vitro lezitshalo ezifana ne-safflower, ifomula elungiselelwe njenge-NAA 2.0 mg/L + 6-BA 4.0 mg/L ingathuthukisa kakhulu izinga lokwakheka kwe-callus futhi ivuselele ukuqina kwehlumela.
