No ka hoʻohana ʻana i nā mea hoʻoponopono ulu ma ke kale

1. Moʻomeheu kikokiko
ʻO ka mea hoʻolalelale hoʻokomo ʻana i ka pua adventitious ʻo MS +4.0mg/L6-Benzylaminopurine (6-BA) +0.1mg/L Naphthalene acetic acid (NAA),a he 1/2MS + 0.4mg/L Naphthalene acetic acid (NAA).
Hoʻohana ʻia ka ʻāpana kumu ma ke ʻano he explant, ʻo ka medium induction callus ʻo MS + 4.0mg /L6-BA + 0.1mg/L 2,4-D, ʻo ke ʻano ʻokoʻa o ka pua ʻo MS + 2.0mg /L. 6-BA + 0.1mg/L Naphthalene acetic acid, a ʻo ke kumu kumu he 1/2MS + 1.0mg/L Naphthalene acetic acid.
Hoʻohana ʻia ka hypocotyl o nā mea kanu sterile ma ke ʻano he explants. He 1/2MS ka mea kanu kanu hua kanu, MS+0.5~1mg/L 6-BA+0.1mg/L NAA, a o ka mea hooulu kumu hooulu he 1/ 2MS+0.2~0.5mg/L Indole Acetic Acid(IAA).

2. Hoʻoikaika i ke aʻa ʻana o nā ʻokiʻoki
Indole-3-Butyric Acid（IBA）/Naphthalene acetic acid(NAA):
Hoʻomoʻu i ke kumu o nā ʻokiʻoki i loko o 10mg/L Indole-3-Butyric Acid（IBA）solution no 2h a i ʻole e komo koke i loko o 100mg/LNaphthalene acetic acid(NAA) solution, a laila ʻoki i loko o ka substrate, hiki ke loaʻa nui. hoʻonui i ka nui o ke aʻa a me ka helu o nā aʻa o nā ʻokiʻoki.

3. E hoʻomalu i ka elongation o nā mea kanu
Paclobutrazol:
I ka wā o ka kanu ʻana i nā mea kanu kale, hiki ke hoʻonui ʻia ka lōʻihi o nā mea kanu, e pili ana i ka hopena hoʻonaninani i ka pae hope. ʻO ka pīpī ʻana i ka mea kanu kale me ka 20mg/LPaclobutrazol solution a i ʻole ka pīpī ʻana i nā lau me ka 40mg/LPaclobutrazol solution i ka wā cotyledon o nā mea kanu plug hiki ke hoʻopōʻino loa i nā mea kanu.

4. E hoʻomalu i ke ʻano o nā mea kanu a hoʻomaikaʻi i nā mea kanu
Daminozide:
Ma hope o ka kanu ʻana i nā mea kanu kale a hoʻomaka ka ulu ʻana o nā mea kanu ma kahi o 1 mahina, e kāpī i nā lau me ka 1000~1500mg/L Daminozide solution hoʻokahi, a laila e pīpī ʻelua ma hope o 1 pule. Hiki i kēia ke pale i ke kiʻekiʻe o ka ulu ʻana o ka kale, hoʻomāhuahua i kona mau kumu, paʻa ke ʻano o ka mea kanu, ʻoi aku ka mānoanoa o nā lau, ʻeleʻele ke kala o ka lau, a ʻoi aku ka lōʻihi o ka wā hoʻonaninani, e hoʻomaikaʻi nui ana i ka waiwai o ka nani.