Kugwiritsa ntchito zowongolera kukula kwa mbewu pa kale

1. Chikhalidwe cha minofu
Njira yodziwika bwino ya bud induction medium ndi MS +4.0mg/L6-Benzylaminopurine (6-BA) +0.1mg/L Naphthalene acetic acid (NAA),ndipo choyikapo mizu ndi 1/2MS + 0.4mg/L Naphthalene acetic acid (NAA).
Gawo la tsinde limagwiritsidwa ntchito ngati chowonjezera, callus induction medium ndi MS + 4.0mg/L6-BA + 0.1mg/L 2,4-D, sing'anga yosiyanitsa masamba ndi MS + 2.0mg/L 6-BA + 0.1mg/L Naphthalene acetic acid, ndi rooting medium ndi 1/2MS + 1.0mg/L Naphthalene acetic acid.
Hypocotyl ya mbande yosabala idagwiritsidwa ntchito ngati zowonjezera. Chikhalidwe chosabala mbande chinali 1/2MS, njira yoyambira yolowera mphukira inali MS+0.5 ~ 1mg/L 6-BA+0.1mg/L NAA, ndipo njira yoyambira mizu inali 1// 2MS+0.2~0.5mg/L Indole Acetic Acid(IAA).

2. Limbikitsani mizu ya cuttings
Indole-3-Butyric Acid (IBA)/Naphthalene acetic acid(NAA):
Zilowerereni m'munsi mwa zodulidwazo mu 10mg/L Indole-3-Butyric Acid (IBA) yankho kwa 2h kapena kuviika mwachangu mu 100mg/LNaphthalene acetic acid(NAA) solution, kenako kudula mu gawo lapansi, lomwe lingathe kwambiri. onjezerani kuchuluka kwa mizu ndi kuchuluka kwa mizu ya cuttings.

3. Yang'anirani kutalika kwa mbande
Paclobutrazol:
Pa mbande kulima kale, ndi elongation wa mbande ndi zambiri zimachitika, amene amakhudza yokongola kwenikweni m`tsogolo siteji. Kupopera mbewu mankhwalawa mbande ndi 20mg/LPaclobutrazol kapena kupopera masamba ndi 40mg/LPaclobutrazol mu nthawi ya cotyledon ya mbande za pulagi kumatha kufupikitsa mbewu.

4. Yang'anirani mawonekedwe a mbewu ndikusintha kukongola kwa mbewu
Daminozide:
Mbeu za kakale zikambidwa ndikuyambanso kukula kwa mwezi umodzi, tsitsani masamba ndi 1000 ~ 1500mg/L yankho la Daminozide kamodzi, kenaka tsitsani kawiri pakatha sabata imodzi. Izi zitha kulepheretsa kukula kwa kakale, kupangitsa kuti zimayambira zikhale zokulirapo, mawonekedwe a chomera chophatikizana, masamba ochulukirapo, mtundu watsamba wakuda, komanso nthawi yokongoletsa nthawi yayitali, kuwongolera kwambiri kukongola.