Tšebeliso ea lilaoli tsa kholo ea limela ho khale
1. Setso sa lisele
Mokhoa o tlang oa ho kenya bud ke MS +4.0mg/L6-Benzylaminopurine (6-BA) +0.1mg/L Naphthalene acetic acid (NAA),'me sebaka sa ho mela ke 1/2MS + 0.4mg/L Naphthalene acetic acid (NAA).
Karolo ea kutu e sebelisoa e le ho kenyelletsa, "callus induction medium" ke MS + 4.0mg/L6-BA + 0.1mg/L 2,4-D, sebaka sa phapang ea bud ke MS + 2.0mg/L. 6-BA + 0.1mg/L Naphthalene acetic acid, 'me sebaka sa metso ke 1/2MS + 1.0mg/L Naphthalene acetic acid.
Hypocotyl ea lipeo tse nyopa e ne e sebelisoa e le li-explant. Mokhoa oa setso oa lipeo o sa hloekang e ne e le 1/2MS, mokhoa oa khale oa ho hlahisa lithunthung e ne e le MS+0.5~1mg/L 6-BA+0.1mg/L NAA, 'me mokhoa oa setso oa metso e ne e le 1/ 2MS+0.2~0.5mg/L Indole Acetic Acid(IAA).
2. Khothalletsa metso ea li-cuttings
Indole-3-Butyric Acid (IBA)/Naphthalene acetic acid(NAA):
Kenya motheo oa li-cuttings ka 10mg/L Indole-3-Butyric Acid (IBA) tharollo bakeng sa lihora tse 2 kapa u kene ka potlako ho 100mg/LNaphthalene acetic acid(NAA), ebe u khaola ka har'a substrate, e ka khonang haholo. eketsa lebelo la metso le palo ea metso ea li-cuttings.
3. Laola bolelele ba lipeo
Paclobutrazol:
Nakong ea temo ea seedling ea khale, ho ka etsahala hore ho be le nako e telele ea lipeo, e amang phello ea mekhabiso nakong ea morao. Ho fafatsa limela tsa khale ka tharollo ea 20mg/LPaclobutrazol kapa ho fafatsa makhasi ka tharollo ea 40mg/LPaclobutrazol nakong ea mokhahlelo oa cotyledon oa lipeo tsa polaka ho ka etsa hore limela li fokotsehe haholo.
4. Laola sebopeho sa semela le ho ntlafatsa mekhabiso ea limela
Daminozide:
Ka mor'a hore lipeo tsa khale li kenngoe 'me limela li qale ho hōla hoo e ka bang khoeli e le' ngoe, fafatsa makhasi ka 1000 ~ 1500mg/L tharollo ea Daminozide hang, ebe u fafatsa habeli ka mor'a beke e le 'ngoe. Sena se ka thibela bolelele ba khōlo ea khale ka mokhoa o sireletsehileng le ka katleho, etsa hore stems ea eona e be e teteaneng, sebopeho sa semela se kopane, makhasi a teteaneng, 'mala oa lekhasi o fifetse, le nako ea mekhabiso e telele, e ntlafatsa haholo boleng ba mekhabiso.
