Kakaretso ea Mekhoa e Tloaelehileng ea Powder ea Rooting
1. Indole-3-Acetic Acid (IAA) le Naphthylacetic Acid (NAA) Rooting Powder
Phofo ea metso e sebelisoang ka ho fetisisa 'marakeng ke motsoako oa 50% oa Indole-3-Acetic Acid (IAA) le Naphthylacetic Acid (NAA) (e nang le 30% IAA le 20% NAA). IAA ke endogenous semela homone e teng ka tlhaho limela; e etsa hore ho thehoe metso ea li-adventitious le ho khothalletsa ho ata ha metso ea lateral. Ha e kena semela, NAA e etsa hore tlhahiso ea ethylene e be teng; maemong a tlase, ethylene ea endogenous e boetse e khothalletsa ho mela. Mokhoa oa kopo: Bakeng sa matokomane le koro, tšoara peo ka motsoako oa 20-30 mg/kg; sena se potlakisa ho mela ha peo le sekhahla sa ho mela ha metso.
2. Indole-3-Butyric Acid (IBA) le Naphthylacetic Acid (NAA) Rooting Powder
Phofo e 'ngoe e tloaelehileng ea metso' marakeng ke motsoako oa 50% oa Indole-3-Butyric Acid (IBA) le NAA (e nang le 40% IBA le 1% NAA). Mofuta ona oa phofo ea metso e hlaha e le phofo e tšoeu ho isa ho e khubelu; E qhibiliha habonolo ka har'a metsoako ea tlhaho, e qhibiliha hanyenyane metsing a futhumetseng, 'me ha e qhibilihe metsing a batang. Motsoako ona o sebelisoa khafetsa ho khothaletsa ho mela ho li-cuttings tsa lifate le lipalesa tsa mekhabiso. Mekhoa ea kopo e kenyelletsa: mokhoa o potlakileng oa ho noa (ho tsepamisa mohopolo oa 500-1000 mg /L, ho kolobisa ka tlaase ho 3-4 cm ea ho itšeha ka metsotsoana e 10-15) le mokhoa o liehang oa ho phunya (ho tsepamisa mohopolo oa 10-100 mg / L, ho kolobisa ka tlaase ho 3-4 cm ea ho itšeha lihora tse 12-4). Limela tse tlaase le nako e khuts'oane li sebelisoa bakeng sa limela tse melang habonolo, ha limela tse ngata li le telele le tse telele li sebelisoa bakeng sa limela tseo ho leng thata ho li metso.
3. Indole-3-acetic acid (IAA) le phofo ea metso ea catechol
Ha li-cuttings tsa English ivy (20-25 cm ka bolelele) li ne li phekoloa ka sehiloeng sa basal le IAA ka bongata ba 10 mg/kg, palo e tloaelehileng ea metso ka ho khaola e ne e le 1.3. Leha ho le joalo, ha motsoako oa IAA (10 mg/kg) le catechol (5 × 10⁻⁵ mol/L) o sebelisoa ho sehiloeng sa basal, palo e tloaelehileng ea metso ka ho khaola e ile ea nyolohela ho 9.4, e bontšang phello e kholo ea synergistic. Lipatlisiso li bontša hore catechol e thibela ka katleho tšebetso ea bioloji ea IAA oxidase ka har'a li-cuttings, kahoo e lumella IAA e sebelisoang ka mokhoa o sa tloaelehang hore e sebelise ka botlalo ts'ebetso ea eona ea ho susumetsa metso le ho fella ka ts'ebetso e phahameng ea metso; ka lehlakoreng le leng, ha catechol e le sieo, IAA e kenang lesela la semela e potlakisa oxidized mme e sa sebetse ke IAA oxidase, e lebisang ho liphello tse mpe tsa metso.
4. Indole-3-acetic acid (IAA) le saccharin
Ho sebelisa lipeo tsa linaoa tsa liphio (2 cm e telele) e le lisebelisoa tsa tlhahlobo, limela li ile tsa kolobisoa ka lihora tse 16 ka tharollo ea 0.02% indole-3-acetic acid (IAA) kapa 0.0019% saccharin (sulfobenzoic acid) pele e fetisetsoa ho sethopo bakeng sa kholo ea hydroponic. Ka mor'a matsatsi a 12 a phekolo, palo ea metso ea lateral e ile ea hlahlojoa; phekolo e nang le 0.02% IAA feela e ile ea fella ka metso ea 42 ka semela, ha phekolo e nang le 0.0019% saccharin feela ha ea ka ea eketsa palo ea metso ea lateral. Leha ho le joalo, tšebeliso e kopantsoeng ea IAA le saccharin (0.02% + 0.0019%) e ile ea fella ka metso e mengata ea 96 ea lateral ka semela, e bontšang phello e hlakileng ea synergistic. Ka mokhoa o ts'oanang, ho sebelisa motsoako oa IAA le saccharin ho lipeo tsa rose ho khothalelitse haholo ho thehoa ha metso ea lateral. Lipatlisiso tse latelang li ile tsa senola hore motsoako ona o khothalletsa ho thehoa ha metso ea lateral ho lipeo tsa limela tse sa tšoaneng tsa temo le tsa temo, hangata li bontša phello ea synergistic.
5. Abscisic acid (ABA) le phofo ea metso ea IAA
Abscisic acid (ABA) e thibela biosynthesis ea gibberellin 'me kahoo e khothalletsa ho mela ha li-cuttings. Ho sebelisa cowpea hypocotyls e le lisebelisoa tsa ho seha, ho phekola motheo oa li-cuttings ka ABA (S-ABA) feela ka likhakanyo tsa 1–5 mg/kg tse khothalelitsoeng ho mela, joalo ka ho inela motheo ho IAA (5 mg/kg) feela. Leha ho le jwalo, tshebediso e kopantsweng ya ABA le IAA (1–5 mg/kg ABA + 5 mg/kg IAA) e bontshitse phello ya synergistic ho hodisetseng metso ka hara ho seha ha cowpea hypocotyl. Ha ABA (1.25–15 mg/kg) e ne e kopantsoe le potassium indole-3-butyrate (K-IBA) kapa IAA (5–25 mg/kg) ho khothalletsa ho mela kapa ho susumetsa kholo ea metso ea limela tse kang lierekisi, tamati, morara le lipopoliri, liphello tsa tlatsetso li ile tsa hlokomeloa maemong a mangata, ka liphello tse itseng tsa synergistic; Leha ho le joalo, ka tsela e feteletseng phahameng concentrations ea ABA inhibited rooting. 6. Tšebeliso e kopanetsoeng ea fulvic acid le potassium indole-butyrate
Tekong e amang li-cuttings tsa apole tse nang le selemo se le seng, ho kenya li-cuttings ka lihora tse 12-24 ka ho hlapolloa ha fulvic acid ka makhetlo a 500 feela ho ile ha khothalletsa ho mela, kaha fulvic acid e susumetsa ts'ebetso ea li-invertase tse itseng. Ho inela lihora tse 12-24 ka potassium indole-butyrate (20 mg/kg) feela le hona ho khothalelitse ho mela. Leha ho le joalo, tšebeliso e kopantsoeng ea fulvic acid le indole-butyrate (dilution ea makhetlo a 500 hammoho le 20 mg/kg) e ile ea eketsa palo ea metso e entsoeng.
Phofo ea metso e sebelisoang ka ho fetisisa 'marakeng ke motsoako oa 50% oa Indole-3-Acetic Acid (IAA) le Naphthylacetic Acid (NAA) (e nang le 30% IAA le 20% NAA). IAA ke endogenous semela homone e teng ka tlhaho limela; e etsa hore ho thehoe metso ea li-adventitious le ho khothalletsa ho ata ha metso ea lateral. Ha e kena semela, NAA e etsa hore tlhahiso ea ethylene e be teng; maemong a tlase, ethylene ea endogenous e boetse e khothalletsa ho mela. Mokhoa oa kopo: Bakeng sa matokomane le koro, tšoara peo ka motsoako oa 20-30 mg/kg; sena se potlakisa ho mela ha peo le sekhahla sa ho mela ha metso.
2. Indole-3-Butyric Acid (IBA) le Naphthylacetic Acid (NAA) Rooting Powder
Phofo e 'ngoe e tloaelehileng ea metso' marakeng ke motsoako oa 50% oa Indole-3-Butyric Acid (IBA) le NAA (e nang le 40% IBA le 1% NAA). Mofuta ona oa phofo ea metso e hlaha e le phofo e tšoeu ho isa ho e khubelu; E qhibiliha habonolo ka har'a metsoako ea tlhaho, e qhibiliha hanyenyane metsing a futhumetseng, 'me ha e qhibilihe metsing a batang. Motsoako ona o sebelisoa khafetsa ho khothaletsa ho mela ho li-cuttings tsa lifate le lipalesa tsa mekhabiso. Mekhoa ea kopo e kenyelletsa: mokhoa o potlakileng oa ho noa (ho tsepamisa mohopolo oa 500-1000 mg /L, ho kolobisa ka tlaase ho 3-4 cm ea ho itšeha ka metsotsoana e 10-15) le mokhoa o liehang oa ho phunya (ho tsepamisa mohopolo oa 10-100 mg / L, ho kolobisa ka tlaase ho 3-4 cm ea ho itšeha lihora tse 12-4). Limela tse tlaase le nako e khuts'oane li sebelisoa bakeng sa limela tse melang habonolo, ha limela tse ngata li le telele le tse telele li sebelisoa bakeng sa limela tseo ho leng thata ho li metso.
3. Indole-3-acetic acid (IAA) le phofo ea metso ea catechol
Ha li-cuttings tsa English ivy (20-25 cm ka bolelele) li ne li phekoloa ka sehiloeng sa basal le IAA ka bongata ba 10 mg/kg, palo e tloaelehileng ea metso ka ho khaola e ne e le 1.3. Leha ho le joalo, ha motsoako oa IAA (10 mg/kg) le catechol (5 × 10⁻⁵ mol/L) o sebelisoa ho sehiloeng sa basal, palo e tloaelehileng ea metso ka ho khaola e ile ea nyolohela ho 9.4, e bontšang phello e kholo ea synergistic. Lipatlisiso li bontša hore catechol e thibela ka katleho tšebetso ea bioloji ea IAA oxidase ka har'a li-cuttings, kahoo e lumella IAA e sebelisoang ka mokhoa o sa tloaelehang hore e sebelise ka botlalo ts'ebetso ea eona ea ho susumetsa metso le ho fella ka ts'ebetso e phahameng ea metso; ka lehlakoreng le leng, ha catechol e le sieo, IAA e kenang lesela la semela e potlakisa oxidized mme e sa sebetse ke IAA oxidase, e lebisang ho liphello tse mpe tsa metso.
4. Indole-3-acetic acid (IAA) le saccharin
Ho sebelisa lipeo tsa linaoa tsa liphio (2 cm e telele) e le lisebelisoa tsa tlhahlobo, limela li ile tsa kolobisoa ka lihora tse 16 ka tharollo ea 0.02% indole-3-acetic acid (IAA) kapa 0.0019% saccharin (sulfobenzoic acid) pele e fetisetsoa ho sethopo bakeng sa kholo ea hydroponic. Ka mor'a matsatsi a 12 a phekolo, palo ea metso ea lateral e ile ea hlahlojoa; phekolo e nang le 0.02% IAA feela e ile ea fella ka metso ea 42 ka semela, ha phekolo e nang le 0.0019% saccharin feela ha ea ka ea eketsa palo ea metso ea lateral. Leha ho le joalo, tšebeliso e kopantsoeng ea IAA le saccharin (0.02% + 0.0019%) e ile ea fella ka metso e mengata ea 96 ea lateral ka semela, e bontšang phello e hlakileng ea synergistic. Ka mokhoa o ts'oanang, ho sebelisa motsoako oa IAA le saccharin ho lipeo tsa rose ho khothalelitse haholo ho thehoa ha metso ea lateral. Lipatlisiso tse latelang li ile tsa senola hore motsoako ona o khothalletsa ho thehoa ha metso ea lateral ho lipeo tsa limela tse sa tšoaneng tsa temo le tsa temo, hangata li bontša phello ea synergistic.
5. Abscisic acid (ABA) le phofo ea metso ea IAA
Abscisic acid (ABA) e thibela biosynthesis ea gibberellin 'me kahoo e khothalletsa ho mela ha li-cuttings. Ho sebelisa cowpea hypocotyls e le lisebelisoa tsa ho seha, ho phekola motheo oa li-cuttings ka ABA (S-ABA) feela ka likhakanyo tsa 1–5 mg/kg tse khothalelitsoeng ho mela, joalo ka ho inela motheo ho IAA (5 mg/kg) feela. Leha ho le jwalo, tshebediso e kopantsweng ya ABA le IAA (1–5 mg/kg ABA + 5 mg/kg IAA) e bontshitse phello ya synergistic ho hodisetseng metso ka hara ho seha ha cowpea hypocotyl. Ha ABA (1.25–15 mg/kg) e ne e kopantsoe le potassium indole-3-butyrate (K-IBA) kapa IAA (5–25 mg/kg) ho khothalletsa ho mela kapa ho susumetsa kholo ea metso ea limela tse kang lierekisi, tamati, morara le lipopoliri, liphello tsa tlatsetso li ile tsa hlokomeloa maemong a mangata, ka liphello tse itseng tsa synergistic; Leha ho le joalo, ka tsela e feteletseng phahameng concentrations ea ABA inhibited rooting. 6. Tšebeliso e kopanetsoeng ea fulvic acid le potassium indole-butyrate
Tekong e amang li-cuttings tsa apole tse nang le selemo se le seng, ho kenya li-cuttings ka lihora tse 12-24 ka ho hlapolloa ha fulvic acid ka makhetlo a 500 feela ho ile ha khothalletsa ho mela, kaha fulvic acid e susumetsa ts'ebetso ea li-invertase tse itseng. Ho inela lihora tse 12-24 ka potassium indole-butyrate (20 mg/kg) feela le hona ho khothalelitse ho mela. Leha ho le joalo, tšebeliso e kopantsoeng ea fulvic acid le indole-butyrate (dilution ea makhetlo a 500 hammoho le 20 mg/kg) e ile ea eketsa palo ea metso e entsoeng.
