Ukusetyenziswa kwezilawuli zokukhula kwezityalo kwikale
1. Inkcubeko yezicubu
I-adventitious bud induction medium yi-MS +4.0mg/LI-6-Benzylaminopurine (6-BA) +0.1mg/L I-Naphthalene acetic acid (NAA),kunye ne-rooting medium yi-1/2MS + 0.4mg/L Naphthalene acetic acid (NAA).
Icandelo le-stem lisetyenziswe njenge-explant, i-callus induction medium yi-MS + 4.0mg/L6-BA + 0.1mg/L 2,4-D, i-bud differentiation medium yi-MS + 2.0mg/L I-6-BA + 0.1mg/L I-Naphthalene acetic acid, kunye ne-rooting medium yi-1/2MS + 1.0mg/L Naphthalene acetic acid.
I-hypocotyl yezithole ezinyumba yayisetyenziswa njengezityalo. Indlela yokulima izithole yayiyi-1/2MS, i-adventitious bud induction culture medium yayiyi-MS+0.5~1mg/L 6-BA+0.1mg/L NAA, kunye ne-rootingculture medium yi-1/ 2MS+0.2~0.5mg/L Indole Acetic Acid(IAA).
2. Ukukhuthaza ukunqunyulwa kweengcambu
Indole-3-Butyric Acid (IBA)/Naphthalene acetic acid(NAA):
Gxuma isiseko se-cuttings kwi-10mg/L Indole-3-Butyric Acid (IBA) isisombululo se-2h okanye ngokukhawuleza udibanise kwi-100mg/LNaphthalene acetic acid (NAA) isisombululo, kwaye emva koko usike kwi-substrate, enokuthi kakhulu ukwandisa izinga leengcambu kunye nenani leengcambu ze-cuttings.
3. Lawula ukwanda kwezithole
Paclobutrazol:
Ngexesha lokulinywa kwezithole kwi-kale, ukwanda kwezithole kunokwenzeka kakhulu, okuchaphazela umphumo wokuhlobisa kwinqanaba elizayo. Ukutshiza i-kale seedling medium nge-20mg/LPaclobutrazol isisombululo okanye ukutshiza amagqabi nge-40mg/LPaclobutrazol isisombululo ngexesha le-cotyledon yezithole zeplagi zinokuzenza zincinci kakhulu izityalo.
4. Lawula ukumila kwezityalo kunye nokuphucula ukuhombisa izityalo
I-Daminozide:
Emva kokuba izithole ze-kale zigalelwe kwaye izityalo ziphinde zikhule malunga nenyanga enye, fafaza amagqabi nge-1000 ~ 1500mg/L isisombululo se-Daminozide kube kanye, kwaye utshize kabini emva kweveki enye. Oku kunokunqanda ngokukhuselekileyo nangempumelelo ukuphakama kokukhula kwe-kale, yenza iziqu zayo zibe ngqindilili, isityalo sokumila sihlanganisene, amagqabi atyebileyo, umbala wegqabi ube mnyama, kunye nexesha lokuhombisa ixesha elide, ukuphucula kakhulu ixabiso lokuhombisa.
