Ukusetshenziswa kwezilawuli zokukhula kwezitshalo ku-kale
1. Isiko lezicubu
I-adventitious bud induction medium yi-MS +4.0mg/L6-Benzylaminopurine (6-BA) +0.1mg/L I-Naphthalene acetic acid (NAA),futhi i-rooting medium ingu-1/2MS + 0.4mg/L I-Naphthalene acetic acid (NAA).
Ingxenye yesiqu isetshenziswa njenge-explant, i-callus induction medium yi-MS + 4.0mg/L6-BA + 0.1mg/L 2,4-D, i-bud differentiation medium yi-MS + 2.0mg/L 6-BA + 0.1mg/L I-Naphthalene acetic acid, futhi indawo yokufaka izimpande ingu-1/2MS + 1.0mg/L I-Naphthalene acetic acid.
I-hypocotyl yezithombo eziyinyumba yayisetshenziswa njenge-explants. Indlela yokutshala izithombo eyinyumba yayiyi-1/2MS, i-adventitious bud induction culture medium kwakuyi-MS+0.5~1mg/L 6-BA+0.1mg/L NAA, futhi isiko lokugxilisa izimpande lalingu-1/ 2MS+0.2~0.5mg/L Indole Acetic Acid(IAA).
2. Khuthaza ukuqothulwa kwama-cuttings
I-Indole-3-Butyric Acid (IBA)/Naphthalene acetic acid(NAA):
Gcoba isisekelo sokusikwa ku-10mg/L Indole-3-Butyric Acid (IBA) isixazululo amahora angu-2 noma ngokushesha cwilisa ku-100mg/LNaphthalene acetic acid(NAA) solution, bese usike ku-substrate, engakwazi kakhulu ukwandisa izinga lezimpande kanye nenani lezimpande ze-cuttings.
3. Lawula ukunwebeka kwezithombo
I-Paclobutrazol:
Ngesikhathi sokutshalwa kwezithombo ze-kale, ukunwetshwa kwezithombo kungenzeka kakhulu, okuthinta umphumela wokuhlobisa esigabeni sakamuva. Ukufafaza isithombo se-kale ngesisombululo esingu-20mg/LPaclobutrazol noma ukufutha amahlamvu ngesixazululo esingu-40mg/LPaclobutrazol ngesikhathi sesigaba se-cotyledon sezithombo zepulaki kunganciphisa kakhulu izitshalo.
4. Lawula ukuma kwesitshalo futhi uthuthukise ukuhlobisa kwezitshalo
I-Daminozide:
Ngemva kokuba izithombo ze-kale sezifakwe ebhodweni futhi izitshalo ziqala ukukhula futhi isikhathi esingangenyanga eyodwa, fafaza amaqabunga ngesisombululo se-Daminozide engu-1000~1500mg/L kanye, bese ufafaza kabili ngemva kwesonto eli-1. Lokhu kungavimba ngokuphepha nangempumelelo ukukhula kwe-kale, kwenze iziqu zayo zibe ukuqina, isimo sesitshalo sihlangane, amaqabunga abe mkhulu, umbala weqabunga ube mnyama, kanye nenkathi yokuhlobisa ibe yinde, ithuthukise kakhulu inani lokuhlobisa.
