Umehluko kanye nokusetshenziswa kwe-zeatin trans-zeatin kanye ne-trans-zeatin riciside

Zeatin (Zt):I-Zeatin igqugquzela ukuhlukaniswa kwamaseli futhi ingathinta ezinye izigaba zomjikelezo weseli. Imisebenzi yayo ifaka phakathi ukuvikela i-chlorophyll kanye ne-protein devalation, ukunciphisa ukuphefumula, ukulondolozela ubungqabavu seseli, ukumbumbuluzwa kwesitshalo se-censecence, kubuyisela imiphumela enobuthi emaqabungeni, kuvimbela ukwakheka kwezimpande, nokukhuthaza ukwakheka kwe-thwebula ngokugxila okuphezulu.

I-Trans-Zeatin (Tz):Inezinhlobonhlobo zemisebenzi, ukukhuthaza ukuhlukaniswa kwamaseli we-microbial kanye nokukhula kwezithelo ezilingweni zezitshalo, kukhombisa imiphumela ebanzi yokukhula kokukhula.

I-Trans-Zeatin Ribiside (TZR):Futhi kuthuthukisa ukukhula kwe-lateral bud, kuvuselela umehluko weseli, kuthuthukisa i-callus kanye nokuhluma kwembewu, kuvimbela insescence yeqabunga, ibuyisa umonakalo odabukisayo.

Imisebenzi eyinhloko

Zeatin, Zt:

1. Ikhuthaza ukuhlukaniswa kweseli, ngokuyinhloko ukwahlukaniswa kwe-cytoplasmic;

2. Ithuthukisa umehluko we-bud; emasikweni izicubu, axhumana ne-auxin ukulawula impande kanye nokwehlukahlukana kwe-bud;

I-3. Ikhuthaza ukukhula kwe-lateral bud, ukuqeda ukubusa okungokoqobo, okuholela enanini elikhulu lama-buds anentshebe emasikweni wezicubu;

4. Ukubambezeleka kweqabunga le-sense, wehlisa izinga lokuwohloka kwe-chlorophyll namaprotheni;

5. Uphula umsele wembewu, uthathe indawo yokukhanya ukuze ahlangabezane nezidingo zembewu efunwa ukukhanya njengogwayi;

6. I-Parthenocarpy kwezinye izithelo;

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8. Ivuselela ukwakheka kwe-potato tuber.

I-Trans-Zeatin, i-TZ: iqukethe kuphela isakhiwo se-trans, nomsebenzi ofanayo ne-Zeatin, kepha ngomsebenzi onamandla.

I-Trans-Zeatin Ribiside, i-TZR: Imiphumela yayo ifana kakhulu ne-Trans-Zeatin, TZ, hhayi kuphela imiphumela ye-Zeatin okukhulunywe ngayo ngenhla, kepha futhi isebenze ukuvezwa kofuzo kanye nomsebenzi we-metabolic.

Ukusetshenziswa:

Zeatin, Zt:

1. Ikhuthaza ukuhluma kwe-callus (kumele isetshenziswe ngokuhlanganiswa ne-auxin), okuhlushwa 1 mg l.

2. Ikhuthaza ukulungiselelwa kwezithelo, zeatin 100 mg l + ga3 500 mg l + nai 201 l, fray on l, fafaza ezinsukwini eziyi-10, 25, kanye nezinsuku ezingama-40 ngemuva kokuqhakaza izimbali.

3. Imifino enamaqabunga, ukufafaza ngo-201 mg l kungabambezela ukuphuzi kwamaqabunga. Ngaphezu kwalokho, ukwelashwa kwezinyembezi zezitshalo kungakhuthaza ukuhluma; Ukwelashwa kwesithombo kukhuthaza ukukhula.


Trans-Zeatin, Tz:

1. Ikhuthaza ukulungiswa kwe-callus (kumele isetshenziswe ngokuhlanganiswa ne-auxin), okuhlushwa 1 ppm;

2. Ikhuthaza ukulungiselelwa kwezithelo, zeatin 100 ppm + ga3 500 ppm + Naa 20 ppm, fafaza ngezithelo 10, 25, kanye nezinsuku ezingama-40 ngemuva kokuqhakaza izimbali;

I-3. Ukubambezeleka okuphuzi kwamaqabunga emifino, fafaza ku-20 ppm;

I-Trans-Zeatin Ribiside (TZR):
1. Kumasiko wezicubu zezitshalo, ukuhlanganiswa okuvame ukusetshenziswa kweTrans-Zeatin Ribiside kuyi-1 mg ml noma ngaphezulu.

2. Ekuqondisweni kokukhula kwezitshalo, ukugcwaliseka kwe-Trans-Zeatin Ribiside ngokujwayelekile i-1 PPM kuya ku-100 ppm, kuya ngohlelo oluthile kanye nezinhlobo ezithile zezitshalo. Isibonelo, lapho kukhuthaza ukuhluma kwe-callus, ukugxila kwe-1 ppm kuyasetshenziswa, futhi kudinga ukusetshenziselwa ukuhlangana nama-auxins.

3. Hlaza i-Trans-Zeatin Riabiside Powder ngo-2-5 ml we-1 m naoh (noma i-1 m acetic acid noma amanzi we-ultrappred ukulungiselela indawo yokuhlunga i-1 mg I-Aliquot isixazululo sesitoko futhi iqhwa, igweme imijikelezo ephindaphindwayo ye-freeze-thaw. Dlulisa isixazululo sesitoko ngokugxila okudingekayo usebenzisa amasiko aphakathi. Lungiselela ikhambi elisebenzayo lisha isikhathi ngasinye.


Izicelo:
Zeatin (zt): Kusetshenziswa kabanzi emasikweni wezicubu zezitshalo kanye nokutshala kwezitshalo njengoMlawuli Wokukhula Kwesitshalo ukukhuthaza ukukhula kwezitshalo nentuthuko.

I-Trans-Zeatin (Tz): Kusetshenziswe kabanzi ekucwaningweni kwesayensi nasekutshalweni kwezitshalo ngenxa ye-bioactivity yayo ebanzi, elungele izidingo ezahlukahlukene zokulawulwa kwezitshalo.

I-Trans-Zeatin Ribiside (TZR): Udlala indima ebalulekile emthethweni wokukhula kwezitshalo futhi wasetshenziswa kabanzi ocwaningweni lwesayensi nasekukhiqizweni kwezolimo.