Kushandiswa kwezvinogadzirisa kukura kwechirimwa pane kare
1. Tsika yetishu
Iyo adventitious bud induction medium ndeye MS +4.0mg/L6-Benzylaminopurine (6-BA) +0.1mg/L Naphthalene acetic acid (NAA),uye midzi yemidzi ndeye 1/2MS + 0.4mg/L Naphthalene acetic acid (NAA).
Chikamu chedzinde chinoshandiswa sekuwedzera, callus induction medium is MS + 4.0mg/L6-BA + 0.1mg/L 2,4-D, bud differentiation medium is MS + 2.0mg/L 6-BA + 0.1mg/L Naphthalene acetic acid, uye midzi yemidzi ndeye 1/2MS + 1.0mg/L Naphthalene acetic acid.
Iyo hypocotyl yembeu isina mbereko yakashandiswa sekuwedzera. Mbeu isingabereki tsika yepakati yaive 1/2MS, adventitious bud induction culture medium yaive MS+0.5~1mg/L 6-BA+0.1mg/L NAA, uye midzi yemidzimu yemidzi yaive 1// 2MS+0.2~0.5mg/L Indole Acetic Acid(IAA).
2. Kurudzira midzi yekucheka
Indole-3-Butyric Acid (IBA)/Naphthalene acetic acid(NAA):
Nyura hwaro hwezvichekwa mu10mg/L Indole-3-Butyric Acid (IBA) mhinduro kwemaawa maviri kana kukurumidza kunyura mu100mg/LNaphthalene acetic acid(NAA) mhinduro, wozocheka musubstrate, inogona zvakanyanya. kuwedzera midzi yemidzi uye nhamba yemidzi yekucheka.
3. Dzora kurebesa kwembesa
Paclobutrazol:
Munguva yekurimwa kwembesa yekare, kurebesa kwembesa kunowanzoitika, izvo zvinokanganisa maitiro ekushongedza mune inotevera nhanho. Kupfapfaidza mbesa dzekare ne20mg/LPaclobutrazol mushonga kana kupfapfaidza mashizha ne40mg/LPaclobutrazol mushonga panguva yecotyledon yemadzinde eplug kunogona kurebesa zvirimwa.
4. Kudzora chimiro chechirimwa uye kuvandudza chirimwa chekushongedza
Daminozide:
Mushure mekunge mbesa dzekare dzaiswa muhari uye mbesa dzinotangazve kukura kweinenge mwedzi umwe, pfapfaidza mashizha ne1000 ~ 1500mg/L Daminozide solution kamwe, wozomwaya kaviri mushure mesvondo rimwe. Izvi zvinogona kudzivirira kureba kwekare zvakachengeteka uye zvinobudirira, kuita kuti madzinde ayo awedzere, chimiro chechirimwa chakakora, mashizha akakora, ruvara rweshizha kusviba, uye nguva yekushongedza kwenguva refu, ichivandudza zvakanyanya kukosha kwekushongedza.
